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Image Search Results
Journal: ACS Nano
Article Title: A Hybrid Protein–Polymer Nanoworm Potentiates Apoptosis Better than a Monoclonal Antibody
doi: 10.1021/nn403973g
Figure Lengend Snippet: Purified scFv-A192 forms nanoparticles whose secondary structure is stabilized by renaturation. (a) Purified scFv-A192 on a 4–20% SDS-PAGE shows a MW of ∼99.6 kDa and a purity of 91.4 ± 1.3%. Outlined lanes were used to determine protein purity using ImageJ. (b) scFv-A192 assembles nanoparticles with a hydrodynamic radius of 85.7 ± 16.5 nm. (c) Changes in protein structure during refolding were characterized by circular dichroism, which show a significant shift in spectra at 190 and 220 nm. (d) Deconvolution of the curves showed a substantial decrease in β-turn 2 content. The lowering of the β-turn content suggests a change in the ELP secondary structure.
Article Snippet: Precast 4–20%
Techniques: Purification, SDS Page, Circular Dichroism